Tonicity induced changes in volume and refractive index of suspended cells quantified with digital holographic microscopy

Björn Kemper, Jens Klokkers, Sabine Przibilla, Angelika Vollmer, Steffi Ketelhut, Gert von Bally, Hermann-Joseph Pavenstädt, Eberhard Schlatter, Bayram Edemir

Abstract


The analysis of changes of the volume and the intracellular solute concentrations of biological cells represents an important topic in cytometry. We present results from a comparative study in which the cell volume and the refractive index of living rat inner medullary collecting duct cells and human pancreatic tumor cells was determined in media with different tonicity by using quantitative digital holographic phase microscopy. The results show that the inverse cell volume correlates linearly with the integral cellular refractive index which is directly related to the concentration of intracellular solutes.

Full Text: PDF

References
  1. E. K. Hoffmann, O. H. Lambert, S. F. Pedersen, "Physiology of Cell Volume Regulation in Vertebrates", Physiol. Rev. 89, 193 (2009).CrossRef
  2. W. E. Crowe, J. Altamirano, L. Huerto, F. J. Alvarez-Leefmans, "Volume changes in single N1E-115 neuroblastoma cells measured with a fluorescent probe", Neuroscience 69, 283 (1995).CrossRef
  3. K. Maric, B. Wiesner, D. Lorenz, E. Klussmann, T. Betz, W. Rosenthal, "Cell Volume Kinetics of Adherent Epithelial Cells Measured by Laser Scanning Reflection Microscopy: Determination of Water Permeability Changes of Renal Principal Cells", Biophys. J. 80, 1783 (2001).CrossRef
  4. K. A. Poulsen, E. C. Andersen, C. F. Hansen, T. K. Klausen, C. Hougaard, I. H. Lambert, E. K. Hoffmann, "Deregulation of apoptotic volume decrease and ionic movements in multidrug-resistant tumor cells: role of chloride channels", Am. J. Physiol. Cell Physiol. 298, C14 (2010).CrossRef
  5. D. Carl, B. Kemper, G. Wernicke, G. von Bally, "Parameter-Optimized Digital Holographic Microscope for High-Resolution Living-Cell Analysis", Appl. Opt. 43, 6536 (2004).CrossRef
  6. P. Marquet, B. Rappaz, P. J. Magistretti, P. E. Cuche, Y. Emery, T. Colomb, C. Depeursinge, "Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy", Opt. Lett. 30, 468 (2005).CrossRef
  7. C. J. Mann, L. F. Yu, C. M. Lo, M. K. Kim, "High-resolution quantitative phase-contrast microscopy by digital holography", Opt. Express 13, 8693 (2005).CrossRef
  8. B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, G. von Bally, "Investigation of living pancreas tumor cells by digital holographic microscopy", J. Biomed. Opt. 11, 034005 (2006).CrossRef
  9. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, "Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy", Opt. Express 13, 9361-9373 (2005).CrossRef
  10. B. Rappaz, F. Charrière, C. Depeursinge, P.J. Magistretti, P. Marquet, "Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium", Opt. Lett. 33, 744 (2007).CrossRef
  11. B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, J. Schnekenburger, "ntegral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy", J. Biomed. Opt. 12, 054009 (2007).CrossRef
  12. M. Kemmler, M. Fratz, D. Giel, N. Saum, A. Brandenburg, C. Hoffman, "Noninvasive time-dependent cytometry monitoring by digital holography", J. Biomed. Opt., 12, 064002 (2007).CrossRef
  13. S. Kosmeier, B. Kemper, P. Langehanenberg, I. Bredebusch, J. Schnekenburger, A. Bauwens, G. von Bally, "Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy", Proc SPIE 6991 699110 (2008).CrossRef
  14. R. Barer, “Interference microscopy and mass determination,” Nature (London) 169, 366 (1952).CrossRef
  15. F. Charrière, N. Pavillon, T. Colomb, C. Depeursinge, T. J. Heger, E. A. D. Mitchell, P. Marquet, and B. Rappaz, "Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba", Opt. Express 14, 7005-7013 (2006).CrossRef
  16. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, "Tomographic phase microscopy", Nat. Meth. 4, 717-719 (2007).CrossRef
  17. M. Debailleul, V. Georges, B. Simon, R. Morin, O. Haeberlé, "High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples", Opt. Lett. 34, 79-81. (2009).CrossRef
  18. T. Kozacki, R. Krajewski, M. Kujawińska, "Reconstruction of refractive-index distribution in off-axis digital holography optical diffraction tomographic system", Opt. Express, 17, 13758-13767 (2009).CrossRef
  19. J. Klokkers, P. Langehanenberg, B. Kemper, S. Kosmeier S, G. von Bally, C. Riethmuller, F. Wunder, A. Sindic, H. Pavenstädt, E. Schlatter, B. Edemir, "Atrial natriuretic peptide and nitric oxide signaling antagonizes vasopressin-mediated water permeability in inner medullary collecting duct cells", Am. J. Physiol. Renal. Physiol. 297, F693-F703 (2009).CrossRef
  20. B. Kemper, D. Carl, A. Höink, G. von Bally, I. Bredebusch, J. Schnekenburger, "Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells", Proc. SPIE 6191, 61910T (2006).CrossRef
  21. B. Kemper, A. Vollmer, C. Rommel, J. Schnekenburger, G. von Bally, "Simplified approach for quantitative digital holographic phase contrast imaging of living cells", J. Biomed. Opt. 16, 026014 (2011).CrossRef
  22. P. Langehanenberg, B. Kemper, D. Dirksen, G. von Bally, "Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging", Appl. Opt. 47, D176 (2008).CrossRef
  23. B. Rappaz, E. Canob, T. Colomb, J. Kühn, V. Simanis, C. Depeursinge, P. J. Magistretti, P. Marquet, "Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy", J. Biomed. Opt. 14, 034049 (2009).CrossRef
  24. G. Popescu, Y. K. Park, N. Lue, C.-A. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, K. Badizadegan, "Optical imaging of cell mass and growth dynamics", Am. J. Physiol. Cell Physiol. 295, C538 (2008).CrossRef

Full Text:

PDF

We use cookies that are necessary for the website to function and cannot be switched off in our systems. Click here for more information.


Photonics Letters of Poland - A Publication of the Photonics Society of Poland
Published in cooperation with SPIE

ISSN: 2080-2242