Tonicity induced changes in volume and refractive index of suspended cells quantified with digital holographic microscopy

Björn Kemper, Jens Klokkers, Sabine Przibilla, Angelika Vollmer, Steffi Ketelhut, Gert von Bally, Hermann-Joseph Pavenstädt, Eberhard Schlatter, Bayram Edemir


The analysis of changes of the volume and the intracellular solute concentrations of biological cells represents an important topic in cytometry. We present results from a comparative study in which the cell volume and the refractive index of living rat inner medullary collecting duct cells and human pancreatic tumor cells was determined in media with different tonicity by using quantitative digital holographic phase microscopy. The results show that the inverse cell volume correlates linearly with the integral cellular refractive index which is directly related to the concentration of intracellular solutes.

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Photonics Letters of Poland - A Publication of the Photonics Society of Poland
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ISSN: 2080-2242