Slow deformation measurement of biological samples with 3D interferometric microscope

Authors

  • Joanna Schmit Bruker Nano Surfaces Division
  • Jason C Reed California NanoSystems Institute, UCLA

DOI:

https://doi.org/10.4302/photon.%20lett.%20pl.v4i2.318

Abstract

The 3D interference microscope is used to measure the mechanical and structural properties of biological samples. In this paper the pulsation of the eye of the zebrafish embryo and the motion of the neuron is measured. The measurement is often performed on a microsphere placed on a biological sample. The microsphere reflects a strong signal, and by applying a magnetic field the sphere can induce a controlled and varying pressure on the object. The deformation of the object and the motion of the sphere provide information about different mechanical properties of the biological sample. This paper specifically concentrates on the measurement of nanometric deformation at frequencies below 10Hz.

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References

  1. http://www.bruker-axs.com/white_light_interferometric_profilometers.html [Direct link]
  2. J. Schmit, K. Creath, J.C. Wyant, "Surface Profilers, Multiple Wavelength, and White Light Interferometry" in Optical Shop Testing, 667-755 (ed. D. Malacara, John Wiley, Hoboken 2007).
  3. J. Reed, M. Frank, J.J. Troke, J. Schmit, S.Han, M.A. Teitell J.K. Gimzewski, Nanotechnol. 19, 235101 (2008). [CrossRef]
  4. References 1-9 within: J. Reed, S. Ramakrishnan, J.Schmit, J.K. Gimzewski, "Mechanical interferometry of nanoscale motion and mechanical properties of living zebrafish embryos", www.acsnano.org 3(8), 2090 (2009).
  5. J. Reed, S. Ramakrishnan, J. Schmit, J. K. Gimzewski, "Mechanical interferometry of nanoscale motion and mechanical properties of living zebrafish embryos", www.acsnano.org 3(8), 2090 (2009).
  6. J. Schmit, K. Creath, App. Opt. 34(19), 3610 (1995). [CrossRef]
  7. J. Schmit, P. Unruh, E. Novak, "Measurement of object deformation with optical profiler", US Patent 7283250 (2007)

Author Biographies

Joanna Schmit, Bruker Nano Surfaces Division

Senior Staff Optical Engineer

Research and Development Group

Jason C Reed, California NanoSystems Institute, UCLA

California NanoSystems Institute

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Published

2012-06-30

How to Cite

Schmit, J., & Reed, J. C. (2012). Slow deformation measurement of biological samples with 3D interferometric microscope. Photonics Letters of Poland, 4(2), pp. 84‚Äď86. https://doi.org/10.4302/photon. lett. pl.v4i2.318

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Articles