Slow deformation measurement of biological samples with 3D interferometric microscope

Authors

  • Joanna Schmit Bruker Nano Surfaces Division
  • Jason C Reed California NanoSystems Institute, UCLA

DOI:

https://doi.org/10.4302/photon.%20lett.%20pl.v4i2.318

Abstract

The 3D interference microscope is used to measure the mechanical and structural properties of biological samples. In this paper the pulsation of the eye of the zebrafish embryo and the motion of the neuron is measured. The measurement is often performed on a microsphere placed on a biological sample. The microsphere reflects a strong signal, and by applying a magnetic field the sphere can induce a controlled and varying pressure on the object. The deformation of the object and the motion of the sphere provide information about different mechanical properties of the biological sample. This paper specifically concentrates on the measurement of nanometric deformation at frequencies below 10Hz.

Full Text: PDF

References
  1. http://www.bruker-axs.com/white_light_interferometric_profilometers.html [Direct link]
  2. J. Schmit, K. Creath, J.C. Wyant, "Surface Profilers, Multiple Wavelength, and White Light Interferometry" in Optical Shop Testing, 667-755 (ed. D. Malacara, John Wiley, Hoboken 2007).
  3. J. Reed, M. Frank, J.J. Troke, J. Schmit, S.Han, M.A. Teitell J.K. Gimzewski, Nanotechnol. 19, 235101 (2008). [CrossRef]
  4. References 1-9 within: J. Reed, S. Ramakrishnan, J.Schmit, J.K. Gimzewski, "Mechanical interferometry of nanoscale motion and mechanical properties of living zebrafish embryos", www.acsnano.org 3(8), 2090 (2009).
  5. J. Reed, S. Ramakrishnan, J. Schmit, J. K. Gimzewski, "Mechanical interferometry of nanoscale motion and mechanical properties of living zebrafish embryos", www.acsnano.org 3(8), 2090 (2009).
  6. J. Schmit, K. Creath, App. Opt. 34(19), 3610 (1995). [CrossRef]
  7. J. Schmit, P. Unruh, E. Novak, "Measurement of object deformation with optical profiler", US Patent 7283250 (2007)

Author Biographies

Joanna Schmit, Bruker Nano Surfaces Division

Senior Staff Optical Engineer

Research and Development Group

Jason C Reed, California NanoSystems Institute, UCLA

California NanoSystems Institute

Downloads

Additional Files

Published

2012-06-30

How to Cite

[1]
J. Schmit and J. C. Reed, “Slow deformation measurement of biological samples with 3D interferometric microscope”, Photonics Lett. Pol., vol. 4, no. 2, pp. pp. 84–86, Jun. 2012.

Issue

Vol. 4 No. 2 (2012)

Section

Articles

License

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).