Low-cost modular design microscope for measuring fluorescent diamonds


  • Adam Mazikowski Gdańsk University of Technology, Department of Metrology and Optoelectronics
  • Maria Babińska Faculty of Electronics, Telecomunications and Informatics, Gdańsk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdańsk
  • Marta Marszewska Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdańsk




Nowadays, microscopy is a widely used method for analyzing various materials. In our research, we use a developed two-channel fluorescence microscope to measure objects excited with two different wavelengths, e.g. fluorescent diamond powders with nitrogen vacancies. The results obtained were compared with images illuminated with white LED observed using a microscope in the transmission mode. The measurements yielded results with satisfactory resolution and image quality. Considering that the microscope built is a budget design, so it can be a cheaper alternative to commercial devices.

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  1. J.C. Stockert, A. Blázquez-Castro, "Fluorescence Microscopy in Life Sciences" Bentham Science Publishers. ISBN 978-1-68108-519-7 (2017). CrossRef
  2. W.W. Hsiao, Y.Y. Hui, P.C. Tsai, H.C. Chang, "Fluorescent Nanodiamond: A Versatile Tool for Long-Term Cell Tracking, Super-Resolution Imaging, and Nanoscale Temperature Sensing", Acc. Chem. Res. 49(3), 400 (2016). CrossRef
  3. M.B. Amiri Olia, P.S. Donnelly, L.C.L. Hollenberg, P. Mulvaney, D.A. Simpson, "Advances in the Surface Functionalization of Nanodiamonds for Biological Applications: A Review", ACS Appl. Nano Mater. 4(10), 9985 (2021). CrossRef
  4. B.S. Miller, et al. Spin-enhanced nanodiamond biosensing for ultrasensitive diagnostics. Nature 587, 588 (2020). CrossRef
  5. B.H. Oh, K.H. Kim, K.Y. Chung, "Skin Imaging Using Ultrasound Imaging, Optical Coherence Tomography, Confocal Microscopy, and Two-Photon Microscopy in Cutaneous Oncology", Front. Med., Sec. Dermatology, 6 (2019). CrossRef
  6. L. Schmidheini et al. "Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy", Biosensors, 12, 148 (2022). CrossRef
  7. W. Białobrzeska, et al. "Quantitative fluorescent determination of DNA - Ochratoxin a interactions supported by nitrogen-vacancy rich nanodiamonds", J. Mol. Liq., 342 (2021). CrossRef
  8. O.S. Wolfbeis, "Materials for fluorescence-based optical chemical sensors", J. Mater. Chem., 15, 2657 (2005). CrossRef
  9. T. Zhang, et al. "Toward Quantitative Bio-sensing with Nitrogen-Vacancy Center in Diamond". ACS Sens., 6, 2077 (2021). CrossRef
  10. N. Nunn, M. Torelli, G. McGuire, O. Shenderova. "Nanodiamond: A high impact nanomaterial". Curr. Opin. Solid State Mater. Sci. 21(1), 1 (2017). CrossRef
  11. S.J. Yu, M.W. Kang, H.C. Chang, K.M. Chen, Y.C. Yu, "Bright fluorescent nanodiamonds: no photobleaching and low cytotoxicity", J. Am. Chem. Soc. 127(50), 17604 (2005). CrossRef
  12. O. Shenderova et al. "Commercial quantities of ultrasmall fluorescent nanodiamonds containing color centers", Proc. SPIE, 10118, 1011803 (2017). CrossRef
  13. M.H. Alkahtani et al. "Fluorescent nanodiamonds: past, present, and future". Nanophotonics, 7(8), 1423 (2018). CrossRef
  14. M. Głowacki, et al. "Fluorescence of nanodiamond cocktails: pH-induced effects through interactions with comestible liquids", Food chemistry, 381, 132206 (2022). CrossRef
  15. Y. Xue, X. Feng, S.C. Roberts, X. Chen, "Diamond and carbon nanostructures for biomedical applications", Functional Diamond, 1(1), 221 (2021). CrossRef
  16. A. Nagl, S.R. Hemelaar, R. Schirhagl, "Improving surface and defect center chemistry of fluorescent nanodiamonds for imaging purposes-a review", Anal. Bioanal. Chem., 407, 7521 (2015). CrossRef
  17. S. Sengottuvel, et al., "Wide-field magnetometry using nitrogen-vacancy color centers with randomly oriented micro-diamonds", Sci. Rep. 12, 17997 (2022). CrossRef
  18. P. Nowicki, E. Czarniewska, "Nanodiamenty: unikalne nanocząsteczki do zastosowania w biomedycynie i biotechnologii", Postępy Biochem., 65(4) (2019). CrossRef
  19. S.D Subedi, V.V. Fedorov, J. Peppers, D.V. Martyshkin, S.B. Mirov, L. Shao, M. Loncar, "Laser spectroscopic characterization of negatively charged nitrogen-vacancy (NV−) centers in diamond", Opt. Mater. Express 9, 2076 (2019). CrossRef




How to Cite

Adam Mazikowski, M. Babińska, and M. Marszewska, “Low-cost modular design microscope for measuring fluorescent diamonds”, Photonics Lett. Pol., vol. 16, no. 1, pp. 16–18, Apr. 2024.