Elliptical optical tweezers for trapping a red blood cell aggregate
DOI:
https://doi.org/10.4302/photon.%20lett.%20pl.v3i3.224Abstract
Red blood cells (RBCs) and their aggregates scatter light strongly. Optical tweezers facilitate the manipulation of cells and light scattering measurements at the single cell level. We show in this letter the successful trapping of an RBC aggregate with elliptical optical tweezers in a free suspension. Two cylindrical lenses are used to form the trap. The distance between the trapped objects and the bottom of the cuvette had a clear effect on image quality. Rotating the cylindrical lenses changes the orientation of the aggregate, which produces a visible change in the light scattering intensity images taken by a CCD camera.Full Text: PDF
References:
- A. Roggan, M. Friebel, K. Dörschel, A. Hahn, G. Müller, "Optical Properties of Circulating Human Blood in the Wavelength Range 400?2500 nm", J. Biomed. Opt. 4, 36 (1999).[CrossRef]
- A.N. Shvalov, J.T. Soini, A.V. Chernyshev, P.A. Tarasov, E. Soini, V.P. Maltsev, "Light-Scattering Properties of Individual Erythrocytes", Appl. Opt. 38, 230 (1999).[CrossRef]
- A.V. Priezzhev, O.M. Ryaboshapka, N.N. Firsov, I.V. Sirko, "Aggregation and Disaggregation of Erythrocytes in Whole Blood: Study by Backscattering Technique", J. Biomed. Opt. 4, 76 (1999).[CrossRef]
- V.V. Tuchin, X. Xu, R.K. Wang, "Dynamic Optical Coherence Tomography in Studies of Optical Clearing, Sedimentation, and Aggregation of Immersed Blood", Appl. Opt. 41, 258 (2002).[CrossRef]
- X. Xu, R.K. Wang, J.B. Elder, V.V. Tuchin, "Effect of dextran-induced changes in refractive index and aggregation on optical properties of whole blood", Phys. Med. Biol. 48, 1205 (2003).[CrossRef]
- V.V. Tuchin, Laser Phys. 15, 1109 (2005)
- A.N. Bashkatov, D.M. Zhestkov, ?.A. Genina, V.V. Tuchin, "Immersion clearing of human blood in the visible and near-infrared spectral regions", Opt. Spect. 98, 638 (2005).[CrossRef]
- K.A. Sem'yanov, P.A. Tarasov, J.T. Soini, A.K. Petrov, V.P. Maltsev, "Calibration-Free Method to Determine the Size and Hemoglobin Concentration of Individual Red Blood Cells from Light Scattering", Appl. Opt. 39, 5884 (2000). [CrossRef]
- M.A. Yurkin, K.A. Semyanov, P.A. Tarasov, A.V. Chernyshev, A.G. Hoekstra, V.P. Maltsev, "Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and a discrete dipole approximation", Appl. Opt. 44, 5249 (2005). [CrossRef]
- D.H. Tycko, M.H. Metz, E.A. Epstein, A. Grinbaum, "Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration", Appl. Opt. 24, 1355 (1985).[CrossRef]
- S.V. Tsinopoulos, D. Polyzos, "Scattering of He-Ne Laser Light by an Average-Sized Red Blood Cell", Appl. Opt. 38, 5499 (1999).[CrossRef]
- A.M.K. Nilsson, P. Alsholm, A. Karlsson, S. Andersson-Engels, "T-Matrix Computations of Light Scattering by Red Blood Cells", Appl. Opt. 37, 2735 (1998).[CrossRef]
- M. Friebel, J. Helfmann, M.C. Meinke, "Influence of osmolarity on the optical properties of human erythrocytes", J. Biomed. Opt. 15, 055005 (2010). [CrossRef]
- A.K. Amerov, J. Chen, G.W. Small, M.A. Arnold, "Article Scattering and Absorption Effects in the Determination of Glucose in Whole Blood by Near-Infrared Spectroscopy", Anal. Chem. 77, 4587 (2005). [CrossRef]
- C.V.L. Pop, S. Neamtu, "Aggregation of red blood cells in suspension: study by light-scattering technique at small angles", J. Biomed. Opt. 13, 041308 (2008). [CrossRef]
- S.V. Tsinopoulos, E.J. Sellountos, D. Polyzos, "Light Scattering by Aggregated Red Blood Cells", Appl. Opt. 41, 1408 (2002). [CrossRef]
- P.J.H. Bronkhorst, J. Grimbergen, G.J. Brakenhoff, R.M. Heethaar, J.J. Sixma, "The mechanism of red cell (dis)aggregation investigated by means of direct cell manipulation using multiple optical trapping", British J. Haematol. 96, 256 (1997). [CrossRef]
- M.D. Khokhlova et al., "Peculiarities of RBC aggregation studied by double trap optical tweezers", Proc. SPIE 7715, 77150M (2010). [CrossRef]
- J.V. Kruchenok, S.B. Bushuk, G.I. Kurilo, N.A. Nemkovich, A.N. Rubinov, "Orientation of Red Blood Cells and Rouleaux Disaggregation in Interference Laser Fields", J. Biol. Phys. 31, 73 (2005). [CrossRef]
- A. Kauppila, M. Kinnunen, A. Karmenyan, R. Myllylä, manuscript under preparation.
- M. Collins, A. Kauppila, A. Karmenyan, L. Gajewski, K. Szewczyk, M. Kinnunen, R. Myllylä, "Measurement of light scattering from trapped particles", Proc. SPIE 7376, 737619 (2010). [CrossRef]
- W.H. Wright, G.J. Sonek, M.W. Berns, "Parametric study of the forces on microspheres held by optical tweezers", App. Opt. 33, 1735 (1994). [CrossRef]
- D. Leith, "Drag on Nonspherical Objects", Aerosol Sci. Technol. 6, 153 (1987). [CrossRef]
- E. Loth, "Drag of non-spherical solid particles of regular and irregular shape", Powder Techol. 182, 342 (2008). [CrossRef]
- R.M.P. Doornbos, M. Schaeffer, A.G. Hoekstra, P.M A. Sloot, B.G. de Grooth, J. Greve, "Elastic light-scattering measurements of single biological cells in an optical trap", Appl. Opt. 35, 729 (1996). [CrossRef]
- D. Watson, N. Hagen, J. Diver, P. Marchand, M. Chachisvilis, "Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap", Biophys. J. 87, 1298 (2004). [CrossRef]
- W.H. Wright, G.J. Sonek, Y. Numajiri, M.W. Berns, "Measurement of light scattering from cells using an inverted infrared optical trap", Proc. SPIE 1427, 279 (1991). [CrossRef]
- Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, M.S. Feld, "Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells", J. Biomed. Opt. Lett. 15, 020506 (2010). [CrossRef]
- M. Kinnunen, A. Kauppila, A. Karmenyan, R. Myllylä, "Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level", Biomed. Opt. Express 2, 1803 (2011). [CrossRef]
- G.J. Streekstra, A.G. Hoekstra, E.-J. Nijhof, R.M. Heethaar, "Light scattering by red blood cells in ektacytometry: Fraunhofer versus anomalous diffraction", Appl. Opt. 32, 2266 (1993). [CrossRef]
Downloads
Additional Files
Published
2011-09-29
How to Cite
[1]
A. J. Kauppila, M. Kinnunen, A. Karmenyan, and R. A. Myllylä, “Elliptical optical tweezers for trapping a red blood cell aggregate”, Photonics Lett. Pol., vol. 3, no. 3, pp. pp. 95–97, Sep. 2011.
Issue
Section
Articles